http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8426575-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff26bd06860787b113a8468035b31e14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cea139ad438b9ce7d33159d45b154c6b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f2eb3ebee4c0d2bcf8606efbdc98e18b |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-5254 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-552 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24334 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24362 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-183 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56983 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-12 |
| filingDate | 2011-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92a97c7176729c49b182c8435e24abd5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a03d08228a22cf82ff3590599e295de9 |
| publicationDate | 2013-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-8426575-B2 |
| titleOfInvention | N-linked glycosylation alteration in E1 glycoprotein of classical swine fever virus and novel classical swine fever virus vaccine |
| abstract | E1, along with Erns and E2 is one of the three envelope glycoproteins of Classical Swine Fever Virus (CSFV). Our previous studies indicated that glycosylation status of either E2 or Erns strongly influence viral virulence in swine. Here, we have investigated the role of E1 glycosylation of highly virulent CSFV strain Brescia during infection in the natural host. The three putative glycosylation sites in E1 were modified by site directed mutagenesis of a CSFV Brescia infectious clone (BICv). A panel of virus mutants was obtained and used to investigate whether the removal of putative glycosylation sites in the E1 glycoprotein would affect viral virulence/pathogenesis in swine. We observed that rescue of viable virus was completely impaired by removal of all three putative glycosylation sites in E1. Single mutations of each of the E1 glycosylation sites showed that CSFV amino acid N594 (E1.N3 virus), as well the combined mutation of N500 and N513 (E1.N1N2 virus) resulted in BICv attenuation. Infection of either E1.N1N2 or E1.N3 viruses were able to efficiently protected swine from challenge with virulent BICv at 3 and 28 days post-infection. These results, along with those demonstrating the role of glycosylation of E rns and E2, suggest that manipulation of the pattern of glycosylation could be a useful tool for development of CSF live-attenuated vaccines. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11560804-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11384648-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11732353-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11697879-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11603767-B2 |
| priorityDate | 2008-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 253.