http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8420325-B2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d2e1febe91ca64b5ffbd453ab0d57f4e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_adc3537b5f4a0db8081edcb63d239195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b082fa6af7a8fa59a24ce5083c84e49b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b0fef9627d12fac89b41c12a4fe24cd5 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00 |
filingDate | 2010-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2013-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f664e57bf3130ae4b9a3026665a64b61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e85e04be412db7fa82a0f6ea7ecda074 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d47f5776f89139ac5cdfbf5263e68eea |
publicationDate | 2013-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-8420325-B2 |
titleOfInvention | Thermostable polymerases having altered fidelity and methods of identifying and using same |
abstract | The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3795681-A2 |
priorityDate | 1996-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 318.