| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3d77625451c3066a1c9a475578e417bc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4ecd5182fff55533cf22d7fdeb05cd59
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_de31c4a5601d96615cf18056410cf1e2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b3d528965c41d3c4f4bb1ea6d057de68 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate |
2007-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2012-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_608ff8b74a74d394c69d4fe909d08ea5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_098bf79923084118acf07e635570cf81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c701d42035f229eb33d78e28b764faa |
| publicationDate |
2012-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8329405-B2 |
| titleOfInvention |
Method for detection of mutant alleles combining real time PCR and REMS-PCR |
| abstract |
The invention provides an amplification system for the simultaneous detection of mutant alleles, and identification of the specific mutated sequence. A sample is enriched and simultaneously genotyped by the presence of peptide nucleic acids (PNAs) probes in an homogeneous single tube amplification reaction, by detecting the cleavage of a fluorophore quencher from the 5′-end of PCR products and, concurrently, selecting DNA that includes specific mutations relative to wild type, by employing a thermostable endonuclease that will only cleave an amplicon formed on a mutation bearing template strand. Oligonucleotides and kits for conducting the amplification system are also provided. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2013157885-A1 |
| priorityDate |
2006-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |