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filingDate 2011-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2012-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2012-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-8309321-B2
titleOfInvention Screening method for identifying protease secretion-deficient mutants of microorganisms
abstract A method for identifying a protease secretion deficient strain of a microorganism involves producing a mutant of the microorganism, followed by adding the mutant to gel-filled wells of a microtitration plate, incubating the mutant in the gel-filled wells under conditions call sing the mutant to secrete proteins, separating the mutant from the gel, and measuring activity of a secretion protease of the microorganism in the gel, wherein either (i) the gel is a substrate for the secretion protease or (ii) the gel contains a substrate for the secretion protease.
priorityDate 2005-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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