http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8309305-B2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5a78131b614a8fe252af8c4cf69013c6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_66991daa0413f9cc20aab03da49c0561 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_268be9afa00cf55b5aa72b1612151ecb http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_46431bffae33bacf267f128e514560b8 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2009-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2012-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5c77396c91f7bcfd94d963d0c59115d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a904ff70ef9d459cfcedd7e6148e62c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c447e00cd61724d0f251990c39f8c502 |
publicationDate | 2012-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-8309305-B2 |
titleOfInvention | Method for discriminating between nucleotide sequences of nucleic acids |
abstract | The present invention describes methods for discriminating between nucleotide sequences of first and second nucleic acids, including: providing a reaction solution, including a deoxynucleotide triphosphate, a DNA polymerase with strand displacement ability, a template nucleic acid fragment, a primer, and a mask oligo; incubating the reaction solution, obtaining a polymerase reaction, and producing an amplification product; detecting the amplification product to discriminate between the nucleotide sequences, wherein the primer is complementary to the first nucleic acid, the mask oligo hybridizes to the nucleotide sequence portion of the first and second nucleic acid, wherein the mask oligo is more complementary to the second nucleic acid than to the first nucleic acid, and wherein the mask oligo is not an origin of an elongation reaction with the polymerase, and a primer portion and a mask oligo portion hybridize to the same regions on the first and second nucleic acid. |
priorityDate | 2008-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 118.