http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8299238-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aeee8d3f65d495ab7c77f7132d3b5da2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_14f6e1d296ea6ec0f08178c54620a821 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_630e3d71aa0baecae7e5534821240a33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6f0f3558aa577043fd4c75a89f3ade2d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-743 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5008 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 |
| filingDate | 2010-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2012-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e18b62dcca96153bdc465e97164814c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b719c7553e0f2e7905d24b674afedde1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_51e0e113c7cb0d139027a3d8b0497f13 |
| publicationDate | 2012-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-8299238-B2 |
| titleOfInvention | Steroidogenesis modified cells and methods for screening for endocrine disrupting chemicals |
| abstract | An isolated steroidogenesis modified cell comprising one or more steroid biosynthesis knock down nucleic acid operatively linked to a promoter, wherein the steroid biosynthesis knock down nucleic acid reduces the expression of a gene selected from the group CYP21A2, CYP11A1, CYP17A1, CYP19A1, 3-βHSD1, 3-βHSD2, 17-βHSD1, StAR, HMGR, CYP11B2, CYP11B1, 5α-Reductase 2, SULT1E1, CYP3A4 and UTG1A1, wherein the cell comprises reduced expression of one or more of said genes. The cells are useful for identifying endocrine disruptors. Accordingly, the disclosure includes in a further aspect a screening assay for identifying an endocrine disruptor comprising: a) contacting a cell described herein with a test substance; b) determining a level of at least one steroid or steroidogenic gene mRNA or enzyme activity; wherein a modulation in the level of the at least one steroid or steroidogenic gene mRNA or enzyme activity compared to a control is indicative that the test substance is an endocrine disruptor. |
| priorityDate | 2009-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1009.