http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8298787-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4ccedcd74794b316d68355f3d1739237 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_251a1d9108ee2818e565bee6c744fea7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_52a24331fe7882d8a0801155df05d542 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 |
| filingDate | 2006-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2012-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e56df03c593e5bf20b3f3edbabf4480 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef4bcc7bdb3df25297f4ea8956da5659 |
| publicationDate | 2012-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-8298787-B2 |
| titleOfInvention | Methods for enhancing a secretion efficiency of recombinant foreign protein in yeast expression system |
| abstract | Provided is a method for improving secretion efficiency of a recombinant foreign protein in a yeast expression system. The method includes transforming a yeast host with a recombinant foreign gene construct containing a galactose-inducible promoter, a secretion signal sequence and a gene encoding the foreign protein to construct a transformed yeast strain; and culturing the transformed yeast strain under the condition that the activity of the galactose-inducible promoter is controlled. Improved secretion efficiency of the foreign protein can be achieved by decreasing overexpression-induced insoluble precipitation of the recombinant foreign protein suffered by a conventional galactose-inducible promoter-based yeast expression system, via appropriate control of a level of galactose functioning as an inducer of the galactose-inducible promoter in cells. Due to improved secretion efficiency of the recombinant foreign protein, present invention makes a contribution to improvement in productivity of recombinant foreign proteins in the yeast expression system and reduction in production costs. |
| priorityDate | 2006-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.