http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8278120-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_66922301b078ac490bd637c3d926148a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4120caaf4997cf81d06cd4d834d404eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a7fc19e2de81935368f0efc098501c30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_04bc350e2ed465c09cb9be511591d714 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43595 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-536 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 |
| filingDate | 2007-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2012-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4aadef8949fbc494a8bfb62cc2b67dd8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da7974611d5b00e6ea2e6887cc53caf4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15bb3dc152d5bafcbe2b95ad01c19953 |
| publicationDate | 2012-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-8278120-B2 |
| titleOfInvention | Method of changing fluorescence wavelength of fluorescent protein |
| abstract | The present invention provides: a method of changing the fluorescence wavelength of a GFP-like fluorescent protein from copepod while maintaining recombinant expression efficiency, which comprises identifying a structural factor for determining the fluorescence wavelength thereof in the three-dimensional structure of the protein and modifying amino acid residues associated with the structural factor; and a modified fluorescent protein obtained by applying said method. For example, with regard to a GFP-like fluorescent protein from Chiridius poppei , His 52 in an α helix-like secondary structure: PFLLS H CM GYG FYHF (α 1 47-61) comprising a fluorescent moiety site GYG is replaced with an aromatic amino acid selected from Phe, Tyr and Trp, so as to cause a red shift of the fluorescent peak wavelength; or it is replaced with Ala, Val, Ile, Leu, Gly, Cys, Met, Ser, Thr, or Asp, Asn, Glu or Gln, so as to cause a blue shift of the fluorescence peak wavelength. |
| priorityDate | 2006-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 236.