| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_60a19ea5c35db217660f1daf8008c359
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3de98cf2dd333efa29b5b376a631cbe1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_12030ffbc539df5b155e08d951f57a7c |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-475 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-51
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate |
2008-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2012-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc1f3d4e65e98dce932bd629731f70cc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_321feb5b17bb005795cebcf2fdbcf9b2 |
| publicationDate |
2012-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8187837-B2 |
| titleOfInvention |
Optimized purification process of recombinant growth factor protein |
| abstract |
A process for the production of a purified recombinant GDF-5 related protein in prokaryotes comprises the steps of bacterial cell disruption and inclusion body solubilization to obtain a solubilized monomer of a GDF-5 related protein, said process characterized by a) disruption of bacterial cells with a high pressure homogenizer at a disruption pressure between 800 and 900 bar; and/or b) treatment of the recovered inclusion bodies with a denaturing solubilization buffer comprising L-arginine. |
| priorityDate |
2007-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |