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filingDate 2011-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2012-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-8178335-B2
titleOfInvention Intracellular production of a nuclease
abstract Methods and compositions are provided that relate to obtaining a recombinant DNA and RNA cleaving nuclease. This involves the over-expression of a fusion protein between maltose-binding protein and a truncated nuclease in a soluble form in the cytoplasm of a host cell from which it can be readily extracted.
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