Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_430c89d97f6839f5364904e710af9704 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c57dbfba3ea0cf3934ad4e7b950dc753 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_30867a0dc9b54d4274938dad6979e0de |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-137 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-125 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2007-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2012-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_857f9daabbc7d837afb952815b5af293 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c46af4d6da52bb2e324ab585a109095 |
publicationDate |
2012-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-8148079-B2 |
titleOfInvention |
Method of producing amplification product by PCR and usage thereof |
abstract |
A method of producing a PCR amplification product is provided that suppresses an effect of precipitate, turbidity, or the like derived from a whole blood sample on a detection in the detection of an amplified nucleic acid by an optical unit. The amplification product complementary to a target nucleic acid in the whole blood sample is produced by PCR in a condition where a ratio of the whole blood sample in a PCR reaction solution is in the range of 0.1 to 0.9% by volume or 0.01 to 1.8 g/L in term of hemoglobin content. When the PCR is carried out with such conditions, even with an untreated whole blood sample, a monitoring of the amplification product by the optical unit can be done while suppressing the effect of the precipitate or the turbidity. |
priorityDate |
2006-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |