| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1e4d19c0142eca419720f96bcf40b66a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_627463f88415418e1aa3e668d1ae91b5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9e9d44c0b47095a71c63780e362e8338
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2abdff9372c4a41797ad87e8bdd4bd64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1d4a5b9a4f1b2318315da82abec06b6e |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0012
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-003 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 |
| filingDate |
2006-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2012-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ef60b3b4bc6d9aa6be8e8386454a3dc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11831eb700fad3bf3b7b8f35696ecd08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e7aaced7984c594d108e0ff502452583
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c48efdcdb58a15adc2216e3a74af2b01 |
| publicationDate |
2012-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8114650-B2 |
| titleOfInvention |
Nucleic acid and its use effecting ciliate tetrahymena bifunctional dihydrofolate reductase-thymidylate synthase deficiency |
| abstract |
A method for producing a ciliate cell with reduced or essentially no dihydrofolate reductase (DHFS) activity or reduced or essentially no thymidylate synthase (TS) activity or both reduced or essentially no dihydrofolate reductase and thymidylate synthase (DHFR-TS) activity is claimed, comprising the steps ofn a) transforming ciliate cells by inserting a construct containing an allele altering the gene encoding the endogenous DHFR-TS into at least one of the endogenous DHFR-TS genes of the ciliate macronucleus (MAC), b) inducing an allelic assortment process in the transformed ciliate cells to generate cells having the construct inserted in most or all functional DHFR-TS genes of the MAC, and c) identifying the cells generated in step b) by cultivation with or without thymidine. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2014295478-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9611503-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10011842-B2 |
| priorityDate |
2005-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |