http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8105835-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6d05a2cc42e17e8ee81e757067fd446e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bbdc68ca0aa38fef2d059853ef2b31e6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_46071c26eafebfb93f4714521d613613 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
| filingDate | 2010-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2012-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d984bf1381a89f4ead85dd4d4870944 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e993e7c77e0def663ec6a492a01cd05e |
| publicationDate | 2012-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-8105835-B2 |
| titleOfInvention | Method for micropropagation of monocots based on sustained totipotent cell cultures |
| abstract | The present invention provides a method of micropropagating a monocotyledonous plant comprising: (a) cultivating an explant of tissue from a monocotyledonous plant shoot tip on a primary medium, wherein the explant has been pretreated with a cold temperature and the primary medium comprises auxin or auxin and cytokinin, to produce a totipotent embryogenic cell culture; (b) treating the totipotent embryonic cell culture with a cold temperature; (c) maintaining the totipotent embryogenic cell culture by cultivation on a secondary medium, whereby a totipotent embryogenic cell culture of a monocotyledonous plant is produced and maintained; and (d) transferring the embryogenic cell culture of step (c) to a tertiary medium to continue multiplication and to produce a plantlet with roots and shoots, thereby micropropagating a monocotyledonous plant. The micropropagation techniques described herein provide plants for such purposes as development of elite plant lines, phytoremediation and biomass production. |
| priorityDate | 2007-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 245.