| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f0956582f6802bcc1d16cb4a7a68906a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb2a7154b080d0a387d31bee57808c10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_34d68be17d2f2950d9b2b16a60ea46b7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1dde994918a8049bf7f8a88b23d27731
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e890ee325d9e1279f2bef1de8d6d3d67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_187f478702704994487ff4d2832f4ba6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc2b03342511abc88f60c63f2acd96d0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c5a8132ee907eb4468a2d6676dbed18b |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N13-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87 |
| filingDate |
2006-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2012-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c27d4214d1c76790515541c5f20b1fbb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35c230b19b0ae8094b59fdbca55779b9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4738edb535708f3f91de54b8f7f98a5a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_46ee03a0b7ed715819f50a6c03abb900
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc00ead8ac9da44c6a170dd604a968ef
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80d35d1fb2411072cb1b5ab6245b8993
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44079399d6551bf103a4603b9c685ced |
| publicationDate |
2012-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8101169-B2 |
| titleOfInvention |
Ocular gene therapy using avalanche-mediated transfection |
| abstract |
The present invention provides a method of treating an ocular disease in a subject. In a first step, a nucleic acid is introduced into cells or a tissue. The nucleic acid is introduced by electron avalanche transfection. With this technique, a high electric field induces a vapor bubble and plasma discharge between an electrode and the surrounding medium. The formation of a vapor bubble generates mechanical stress. Plasma discharge through the ionized vapor in the bubble enables connectivity between the electrode and the surrounding medium, so that mechanical stress and electric field are applied simultaneously, which results in permeabilization of the cells or tissue. This permeabilization in turn allows the nucleic acid to enter the cell or tissue. Cells or tissue containing the nucleic acid are then transplanted into an ocular region of the subject. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021249786-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022234003-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021030251-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3799879-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021074695-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3881860-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019008335-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023057946-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023057567-A1 |
| priorityDate |
2005-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |