http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8097412-B2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4afcdb8188866c51bb7b416482c47cd0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_82a2faeb824c7aebd2289661e596f639 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1a557574eef90652422b32a6dae55770 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 |
filingDate | 2009-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2012-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4dc8af2f235706e8670577c04f630a6d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d5602f1371d88d1bbe338e606042ec9 |
publicationDate | 2012-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-8097412-B2 |
titleOfInvention | DNA-based test for detection of annual and intermediate ryegrass |
abstract | We have developed a novel TaqMan quantitative PCR (Q-PCR) based DNA test for detecting annual and/or intermediate ryegrass types in perennial ryegrass. This DNA test was designed using an insertion/deletion (InDel) site in the LpVRN2_2 gene. The new DNA test is more reliable, accurate, and cost effective in detecting annual and intermediate type contamination in perennial ryegrass, having a sensitivity of 0.04% in a sample size of 5000 seeds. Use of a higher sample size (12.5-fold higher compared to the SRF test) provides additional accuracy in detecting the level of contamination A forward and reverse set of primers also identified an approximately 450 bp fragment in or near the LpVRN1 promoter, the fragment being present for all perennial, but not annual, varieties tested. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103773868-A |
priorityDate | 2008-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 442.