http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8017837-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cef1d7ffaf9ca816bad43f580bc6e496 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2008-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2011-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf911611a8e7e8e124e7fea6189f1af9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c84d4adb52e057b0a31195c944e0654a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_349675259489899438c69b21b773ea62 |
| publicationDate | 2011-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-8017837-B2 |
| titleOfInvention | Method for producing transgenic plants |
| abstract | A method for producing transgenic plants, including treating a target tissue using plasmolyzing media (PM) which contains 4% to 10% of sucrose and 100 μM to 300 μM of Acetosyringone (AS) and gold particles. The target tissue is infected by a bacterial suspension using a suitable strain and a suitable transformation vector. A PM containing 4% to 10% sucrose and 100 μM to 300 μM AS is treated for a period between 1 to 3 days. Cultivation is performed in a cultivation media in a dark condition at a temperature between 25° C. to 30° C. A non-selection media with an antibiotic is introduced. A selection media containing an active ingredient phosphinothricin (PPT) is introduced in a light condition at a temperature of between 25° C. to 30° C. in a sub culture for a period of between 3 weeks to 1 month. The putative transformant is regenerated and the number of copies of the transgenes is analyzed. |
| priorityDate | 2007-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.