| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1c89ce4d9ac03f9acd8ddede1162f94d |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-69
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate |
2005-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2011-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_569361aa349aac99d03c4d0bba98a042
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7e460ed913867e6f24c9fa3c87ef7920
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf0d9c89d4c53347ecbe4dd173cdb083
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6765f4ebb2e5f6c165938da9e0ba18a8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c7147e574220189aaba0d069cdd3fae5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_340272f55b78e0c5a0a6806fd4a34d9c |
| publicationDate |
2011-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-7998732-B2 |
| titleOfInvention |
Process for large scale production of plasmid DNA by E. coli fermentation |
| abstract |
The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of E. coli , including but not limited to the DH5 strain, harboring a DNA plasmid and cultivating said clonal subtype with fed-batch fermentation in a chemically-defined medium. The plasmid DNA production process described herein can generate record quantities of plasmid DNA when said highly productive clonal subtypes are cultivated on an industrial scale. The disclosed method can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9102970-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2013171693-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8765411-B2 |
| priorityDate |
2004-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |