http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7994397-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1023880af10dbe26a57dfb1cb687f92d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8287 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8289 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 |
| filingDate | 2007-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2011-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_23f74fa7be5e5d8e7890d3d17b67b200 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_994d0ea7fa02897991803d13a9be08d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73ee7e1c6d7c5a57ce6165ea10a9eff4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8673d609d74752fc44c6f9214905e634 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_88c921a4496804df72bc70001da36101 |
| publicationDate | 2011-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-7994397-B2 |
| titleOfInvention | Method to produce sterile male flowers and partenocarpic fruits by genetic silencing, associated sequences and vectors containing said sequences |
| abstract | Genes VvPI from Vitis vinifera cv. Cabernet Sauvignon and LePI from Lycopersicon esculentum are described; together with the use of these genes to produce sterile male flowers and seedless or parthenocarpic fruits. Silencing vectors that comprise these sequences or a part thereof are disclosed. The methods of the invention are directed to producing sterile male flowers and parthenocarpic fruits by genetic silencing, and includes: obtaining the codifying sequence of Pistillata (PI)-homologous genes from the target species; analyzing the expression of the sequence obtained in step (a) to test its expression according to the pattern described for Pistillata genes; analyzing the complementation of PI-gene mutant with the PI sequence obtained from the target species, to assess that the obtained sequence fulfills the function of a PI gene; making a genetic silencing construct that comprises a region of the codifying sequence of PI in a plant expression vector; incorporation of the constructed vector into Agrobacterium tumefaciens ; transforming target plants with Agrobacterium tumefaciens modified with the silencing vector and selecting said transformed plants; and checking the absence of Agrobacterium contamination and corroborating transgenic plants by transgene amplification. |
| priorityDate | 2006-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 275.