| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b909a8f57cbd2b163a5fcc4cf7a04be8 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1051 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 |
| filingDate |
2004-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2011-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56e3e3566c7b8d9302c274be7b0515f2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98a91b941432a444c861d01da71030af
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85ad590c57501aaf21159f1059a1bac5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90d5f954b861328d798e6d29c82b03d7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4cda71676614f8b4ab43eb50a5e9bef1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ab2551943550d213ec12cb1e1501a0f |
| publicationDate |
2011-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-7968309-B2 |
| titleOfInvention |
Method for improving the thermostability of sucrose phosphorylase (SP) |
| abstract |
A sucrose phosphorylase (SP) having improved thermostability obtained by modifying a natural SP and a method for producing the SP having improved thermostability is provided. This SP having improved thermostability has an amino acid residue which is different from that of the natural sucrose phosphorylase, in at least one position selected from the group consisting of a position corresponding to position 14, a position corresponding to position 29 and a position corresponding to position 44 in motif sequence 1; a position corresponding to position 7, a position corresponding to position 19, a position corresponding to position 23 and a position corresponding to position 34 in motif sequence 2; and a position corresponding to position 19 in motif sequence 3, and wherein the enzyme activity of the SP having improved thermostability at 37° C., after heating the SP having improved thermostability in 20 mM Tris buffer (pH 7.0) at 55° C. for 20 minutes, is 20% or more of enzyme activity of the SP having improved thermostability at 37° C. before heating. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10131929-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11180740-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10927392-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020023278-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016106011-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10392641-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019209559-A1 |
| priorityDate |
2003-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |