http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7838269-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9019c0ce5cb7b82c85e121f6cac86dce |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6865 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2006-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2010-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e30412e00951d1355e58c6dbc4c65c2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3bfecfdaebf1987f8a503cec83d3959 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9797fcce75b8f9460d64af4d9f7326c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b23f97ecbb36b1b11d43c00d7248a7cc |
| publicationDate | 2010-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-7838269-B2 |
| titleOfInvention | Gene detecting method |
| abstract | Disclosed is a gene detecting method for determining mutation of a specific base or presence/absence of a specific base in a target gene. There are provided a the target gene sample and a control gene sample having a base sequence which is wild-type or standard-type with respect to the target gene. The method comprises steps of (i) independently subjecting the target gene sample and the control gene sample to a PCR reaction for amplification, using primers having an RNA polymerase promoter sequence at the 5′-end thereof, (ii) independently subjecting the double-stranded DNAs produced by said PCR reaction from the target gene sample and from the control gene sample, to an in vitro transcription reaction to form a single-stranded RNA, (iii) independently hybridizing the single-stranded RNAs with a fluorescence-labeled probe composed of a single-stranded DNA having a base sequence complementary to at least part of the base sequence of the control gene and being combined with a fluorescent dye, to form an RNA/DNA hybrid, and then (iv) comparing the fluorescence intensity of the RNA/DNA hybrid derived from the target gene sample with that of the RNA/DNA hybrid derived from the control gene sample. |
| priorityDate | 2005-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.