http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7838230-B2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a88b8ec09e31a28046ea82a2ee8b3e72 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6839 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2005-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2010-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ce9f63267c4609eea361cf7dc8a436a |
publicationDate | 2010-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-7838230-B2 |
titleOfInvention | Assay for measuring an enzyme's capability to modify supercoil topology of nucleic acids and modulators |
abstract | Provided are methods, such as high throughput methods, of assessing or measuring the ability of an enzyme to modify the topology (e.g. supercoil topology) of a target nucleic acid, the methods comprising the steps of: (a) providing a solid support to which a capture nucleic acid is or may be immobilized, which capture nucleic acid is capable of binding the target nucleic acid in a manner that is proportional to the supercoil topology of said target nucleic acid (e.g. by triplex formation); (b) incubating a test mixture comprising (i) the enzyme, (ii) the target nucleic acid, (iii) capture nucleic acid, in the presence of (iv) said solid support, such that supercoiled target nucleic acid bound by the capture nucleic acid is immobilized to the solid support, (c) determining the amount of target nucleic acid bound by said capture nucleic acid in step (b) e.g. by use of a detectable label. The method may be used to screen for modulators of the enzyme activity. |
priorityDate | 2004-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 69.