| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_07c9542186133097bd6bbb7d92393090 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-523 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-24069
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-0258
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-52 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate |
2008-02-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2010-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e5e817cbca5e6208cd9327d6b7868d5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a81aebac8d1ba461c27f72378787eeb5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_822058bc991fba47dffbd0e74d5acaa7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8d45d5977eaff7d3568e5efcb35e42e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3427a848b2bcaa81dbc8c389ea28b20c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91846aecad718ae50201f4b6de24d34d |
| publicationDate |
2010-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-7820411-B2 |
| titleOfInvention |
Escherichia coli-derived vaccine and therapy against botulism |
| abstract |
A method of producing botulinum toxin C-terminal receptor binding domain (HCR) is disclosed. The one embodiment, the method comprises the steps of (a) preparing E. coli transformed with an expression vector comprising DNA encoding HCR protein, (b) inducing expression of the HCR protein at a reduced temperature in a culture media, and (c) purifying the HCR protein via extraction, wherein the extraction comprises a clarification by centrifugation and a filtration, wherein the purified HCR protein is at least 10 mg/L of culture medium. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8153397-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010273211-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010297180-A1 |
| priorityDate |
2004-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |