| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efb35e154fff5d3177ae8520d41046d5 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1013 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-08 |
| filingDate |
2009-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2010-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f778a744e83e8c2309cf451972858217
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d56403486bc0475281d69aaec0b7414
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_645f4f3960fbd82759f6690eed76e141
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6b226b133979a930af290b388e093ed0 |
| publicationDate |
2010-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-7803581-B2 |
| titleOfInvention |
Alkaline shock-based preparation of nucleic acids |
| abstract |
Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8071301-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8420317-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011008847-A1 |
| priorityDate |
2005-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |