| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d07a4ddba85ae3e35ca0a6f85dad9f6d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a490020fd2580d5f04cbe4ea55b65c3f |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-107497
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-108331
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-25
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-25375
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-25125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-2525 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-80 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-00 |
| filingDate |
2004-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2010-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_55977324eae6d320712eb3ec00630231
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7bfa916fbd9bedd0ab7bc132e2d08ef2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba7f65af34881303cc502cd009179972
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9aba506b9afbd6c3352726b9f892d7c0 |
| publicationDate |
2010-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-7803523-B2 |
| titleOfInvention |
Whole blood preparation for cytometric analysis of cell signaling pathways |
| abstract |
This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the invention allows for the rapid fixation of biological samples containing red blood cells, to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in the active state. The method of the invention further allows for lysis of red blood cells, thereby making it a useful method for cytometric analysis of biological samples, including, for example, whole blood, bone marrow aspirates, peritoneal fluids, and other red blood cell containing samples. The invention also provides a method to recover or “unmask” epitopes on intracellular antigens that have been made inaccessible by the cross linking fixative necessary to fix the sample. Significantly, the methods of the invention allow preservation and analysis of phospho-epitope levels in biological samples taken directly from patients to determine disease-specific characteristics. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010086951-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015057402-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2013295582-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8962263-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2018172564-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3360969-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9040249-B2 |
| priorityDate |
2004-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |