http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7781190-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7c3d76288892ddbddca3e1fe2313ec5c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2004-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2010-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2eda3bfcc6e0997ea6c481823e785609 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a7c2353b2edfa689dcbbf00fe588978 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6b2b9ff39cf8391be3353ef5f80409cf |
| publicationDate | 2010-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-7781190-B2 |
| titleOfInvention | Method for constructing and modifying large DNA molecules |
| abstract | This invention provides a method for combining overlapping DNA molecules comprising: (a) providing first and second DNA fragments, the first having a region homologous to a region in the second; (b) tagging the first DNA fragment with a selectable marker; (c) cloning the first DNA sequence into a retrieval vector to form a DNA-vector complex; (d) linearizing the DNA-vector complex; and (e) inserting the first DNA fragment from the DNA-vector complex into the second DNA fragment using homologous recombination to form a combined DNA molecule; and (f) removing the selectable marker, thereby generating a combined DNA molecule. The invention further provides a vector for retrieving and inserting a selected DNA molecule into a target DNA molecule. |
| priorityDate | 2003-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.