http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7741054-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_85b959d2100bb308fcf773d125a37ab8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2400-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-2828 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6896 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54393 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
| filingDate | 2005-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2010-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_089125538718a4ff0fc0adfd6603578a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4fa1cbff15f97b94a356fbc4e298594e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_163ba64b2d5d2d980748472d314edd45 |
| publicationDate | 2010-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-7741054-B2 |
| titleOfInvention | High throughput prion assays |
| abstract | The invention is directed to a method for performing a high throughput test to determine the presence of PrPsc in a tissue sample taken post mortem from brain, the test comprising the workflow of (A) sample preparation, (B) sample treatment, (C) sample analysis, (D) controls, and (E) classifying the results of the analysis as positive or negative. The invention is further directed to preparing a treated biological sample suspected of containing PrPsc such that it is suitable for specific detection of PrPsc, if present, the method comprising the steps of (a) homogenizing the sample; followed by (b) digesting the sample proteolytically by adding to the homogenate Proteinase K and incubating the sample; followed by (c) adding a peptidic protease inhibitor to the incubated mixture of step (b), thereby obtaining a treated biological sample suitable for specific detection of PrPsc, if present. The invention further provides a conditioned microwell plate. In addition, the invention provides a soluble fusion protein comprising one or more epitopes of prion protein as well as a carrier polypeptide. |
| priorityDate | 2004-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 121.