http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7727764-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a5e26b6cd5775da5022cecf450367f12 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0087 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2006-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2010-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fdd0daa9522279d6db5d5d9d733d2efb |
| publicationDate | 2010-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-7727764-B2 |
| titleOfInvention | Non-isopycnic cell purification using percoll |
| abstract | A method of “non-isopycnic” cell isolation and purification has the steps of adding a sample of blood to a defined volume to a corresponding ratio volume of EDTA solution to produce a volume of anti-coagulated blood; taking a predetermined volume of the anti-coagulated blood and placing it in a first tube containing a selected defined volume of PSS, wherein the selected defined volume of PSS is taken from a group of defined volumes of PSS, as each defined volume of PSS establishes a specific cell type to be purified; centrifuging the tube for a first predetermined time and speed to form a volume of supernatant of plasma/PSS and a bottom sedimented volume of mostly red blood cells; extracting the supernatant to within a proximity of an interface between the sedimented red blood cells and the supematant; transferring an appropriate, pre-selected volume amount of the supernatant into a second tube holding a defined volume of physiological media; mixing the solutions gently; centrifuging for a second predetermined speed and time; and pouring off the supernatant wherein at the bottom of the tube will be a cell button containing a volume of the selected purified cells in a high percentage and a very small quantity or low percentage of some contaminating non-selected cells. This method is useful in establishing high purity concentrations of selected cells from whole blood such as monocytes, lymphocytes, neutrophils and basophils. |
| priorityDate | 2006-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.