http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7723528-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_60f0a3d3aed05b92d8e241a056bda6ef |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D417-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D285-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D413-00 |
| filingDate | 2008-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2010-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ddaac7557e8fe371150016dff2187461 |
| publicationDate | 2010-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-7723528-B2 |
| titleOfInvention | Sulfur transfer reagents for oligonucleotide synthesis |
| abstract | The use of N-formamidino-5-amino-3H-1,2,4-dithiazole-3-thiones as novel, efficient sulfur-transfer reagents is disclosed. The sulfur transfer from these reagents to compounds containing P(III) atom, triphenylphosphine, 5′-O-DMT-thymidine 2-cyanoethyl-(N,N-diisopropyl)phosphoramidite, and 5′-O-DMT-3′-O-levulinyl dithymidilyl 2-cyanoethyl phosphite, was studied in solution by 31 P NMR and HPLC. The sulfur transfer from title compounds was also studied in the solid-phase synthesis of oligonucleotide phosphorothioates by phosphoramidite methods. In this application, the efficiency of the sulfur transfer reaction for 2′-deoxyoligonucleotides was better than 99.5%. The novel sulfurizing agents are synthesized, at low cost, using simple chemical methods. As opposed to many sulfur transfer reagents known in the prior art such as 1,2-benzodithiol-3-one-1,1-dioxide (Beaucage reagent) and 5-ethoxy-3H-1,2,4-dithiazole-2-one (EDIT), the sulfurizing agents disclosed herein are highly stable in solution, which increases their practical and commercial value. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11447451-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011137021-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10669301-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8710210-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8552175-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10781175-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10087208-B2 |
| priorityDate | 2008-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 273.