http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7723090-B2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b909a8f57cbd2b163a5fcc4cf7a04be8 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-02 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 |
filingDate | 2009-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2010-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ab2551943550d213ec12cb1e1501a0f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4cda71676614f8b4ab43eb50a5e9bef1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90d5f954b861328d798e6d29c82b03d7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56e3e3566c7b8d9302c274be7b0515f2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98a91b941432a444c861d01da71030af |
publicationDate | 2010-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-7723090-B2 |
titleOfInvention | Method of heat-stabilizing α-glucan phosphorylase (GP) |
abstract | An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating. |
priorityDate | 2003-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 591.