http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7691966-B2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e5ad8562c578b061df7a2c3aee9d0e24 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-02 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6445 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6845 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6875 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6872 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 |
filingDate | 2005-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2010-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_51cf8a0071ff73d80efd8df865548918 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3773a098ae8dbd20df598320a08b66f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af876d72aaa13c7cdcd48609979ffb4f |
publicationDate | 2010-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-7691966-B2 |
titleOfInvention | Method for quantitative analysis for prolyl hydroxylase activity using fluorescence polarization |
abstract | The present invention relates to a method for analyzing the interaction between HIF-1 peptide and VBC protein using fluorescence polarization, more precisely, a method for quantitative analysis of formation of HIF-1-VBC protein complex which is composed of the steps of 1) preparing a fluorescent probe by attaching a fluorescein to hydroxyproline containing HIF-1 peptide; 2) reacting the fluorescent probe with VBC protein; and 3) measuring the fluorescence polarization of the above reactant and then comparing the fluorescence polarization with that of the fluorescent probe itself to investigate the changes of fluorescence polarization; a method for screening an inhibitor of the binding of HIF-1 peptide and VBC protein using the above method; and a method for analyzing the activity of prolyl hydroxylase using the above method. The method of the present invention enables simple analysis of the interaction between HIF-1 peptide and VBC protein by observing changes of fluorescence polarization and thus, it can be effectively used for high-speed screening using a well plate. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015010576-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9766240-B2 |
priorityDate | 2005-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 85.