http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7659078-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3fcb093a174d5182b5854b704cb09354 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-807 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-971 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-975 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-88 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-537 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 |
| filingDate | 2003-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2010-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec308f935eba0ad063e510849abe7e48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec9f98012a32c42bbbe1f797b9316ec7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_170ad71e9c451f696d695ad31fe892cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_342ce5b2cb8a5779897db901bf2cc10e |
| publicationDate | 2010-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-7659078-B1 |
| titleOfInvention | Method and kit for determination of prostacyclin in plasma |
| abstract | A solid-phase immunoassay for 6-keto-Prostaglandin F 1α , the stable hydrolysis product of prostacyclin (Prostaglandin I 2 ) is disclosed. Prostacyclin, a potent vasodilator with anti-platelet and anti-proliferative properties is an effective treatment for primary pulmonary hypertension and pulmonary arterial hypertension associated with scleroderma and scleroderma-like syndrome. Levels of 6-keto-Prostaglandin F 1α can be directly correlated with levels of prostacyclin. Therefore, 6-keto-Prostaglandin F 1α has become the indicator of choice to measure prostacyclin levels. The single step immunoassay for 6-keto-Prostaglandin F 1α uses the bioluminescent protein, aequorin as a label. Analyte-label conjugates were constructed by linking the carboxyl group of 6-keto-Prostaglandin F 1α and lysine residues of aequorin by chemical conjugation methods. The binding properties of 6-keto-Prostaglandin F 1α towards its antibody and the bioluminescent properties of aequorin are retained in the conjugate. The concentration of 6-keto-Prostaglandin F 1α after extraction from plasma shows good correlation with the concentration of 6-keto-Prostaglandin F 1α obtained without prior extraction of the same plasma sample. The assay allows the measurement of 6-keto-Prostaglandin F 1α directly in plasma without any pre-treatment of the samples, which results in a much simpler method with a faster assay time. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11534504-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020072775-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-4015644-A1 |
| priorityDate | 2002-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 98.