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filingDate 2000-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2010-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc5f34409978b4cb1881c574a9ff66f8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d528032985d2276e9abec423d330017f
publicationDate 2010-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-7659054-B1
titleOfInvention Methods for genetic analysis of DNA to detect sequence variances
abstract The presently claimed invention concerns a method for biasing the amplification of the DNA molecules in a sample such that a nucleic acid molecule having a specific nucleotide at a selected position (e.g., a polymorphic site) is preferentially amplified relative to an otherwise identical nucleic acid molecule not having the specific nucleotide at the selected position. The method entails amplification of target nucleic acid molecules using a pair of primers one of which causes the incorporation into the amplification product of a sequence that interferes with amplification of nucleic acid molecules not having the specific nucleotide at the selected position. The method is useful for preferentially amplifying at least a portion of one allele of a gene relative to another, different allele of the gene in a sample containing both alleles of the gene.
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priorityDate 2000-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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