Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0e41fd2c81edaeed29a4bbadc357876f |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-726 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-02 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-705 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate |
2007-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2009-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa042e0aebccc24bd01fa9cb20902ca9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0bd864e30bfa98af20a4a128d4635637 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6dec2aa209aa3e7cb82acf79fc68bacf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_95ccce6d8fd234fa14beda51e234c280 |
publicationDate |
2009-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-7604959-B2 |
titleOfInvention |
Cell-based assays employing voltage and calcium dyes |
abstract |
Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions. |
priorityDate |
2001-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |