http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7566529-B2

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1276
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12
filingDate 2002-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2009-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c27de91f53d5df8ff58e6861731597c3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7416ea99769b3536dbb0a19669fe92d2
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publicationDate 2009-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-7566529-B2
titleOfInvention Method for the recovery of retroviral reverse transcriptase (RT) activity from a sample containing unprotected cellular polymerases
abstract A method of recovering an enzyme activity, such as reverse transcriptase (RT) activity, from enveloped viruses, such as HIV, in a biological sample containing a non-protected enzymes, is described. A cysteine-modifying substance is used to destroy the activity of the non-protected enzymes, followed by removal of the enveloped virus particles or inactivation of the cysteine-modifying substance with a chemical. Then the virus envelope is lysed and the released enzymes are recovered. Commercial packages containing written and/or data carrier instructions and some chemicals for performing laboratory steps for recovery of an enzyme activity from enveloped viruses, are also disclosed.
priorityDate 2001-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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