http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7553643-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_70ae68baf731b7c15eb63246ffa512ef |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N- |
| filingDate | 2006-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2009-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fadcd40cba2e292455d9ec4b667e54ef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9671f4611204990c0363c9c55f0da6ef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a6dfed5f5a69271b3525b3d86a61207a |
| publicationDate | 2009-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-7553643-B2 |
| titleOfInvention | Method for amplifying target nucleic acid sequences using a primer comprising an AP endonuclease-cleavable linker |
| abstract | The present invention provides a novel method for nucleic acid amplification. The method includes contacting the sample with at least one forward primer and at least one reverse primer, an AP endonuclease, and a nucleic acid polymerase. under conditions sufficient to allow the forward and reverse primers to hybridize to the target nucleic acid and form a reaction mixture, wherein at least one of the forward and reverse primer includes an AP endonuclease-cleavable linker L, and incubating the reaction mixture under reaction conditions that simultaneously allow the AP endonuclease to cleave at a linker site L and the extension of the primers in a template-specific manner to amplify the target nucleic acid sequence. |
| priorityDate | 2002-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 534.