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filingDate 2003-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2009-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba9d8e7c9ae9f653ee49e27b6268b0f3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b0f3565a545ea2c39e66c5ae9092e5f
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publicationDate 2009-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-7498481-B2
titleOfInvention Method for obtaining a monocotyledon plant containing a gene of interest free of foreign ancillary sequence
abstract The present invention relates to a method for obtaining a transgenic monocotyledon plant containing a gene of interest free of foreign ancillary sequences comprising:n a) transforming at least one cell of a plant having no active transposase with a vector comprising two expression cassettes, one comprising a nucleotide sequence of interest (i), the other comprising a nucleotide sequence encoding a selection marker (ii) bordered by the mobilizable sequences of a transposon, said first expression cassette (comprising the nucleotide sequence of interest (i)) being outside the transposon element; b) selecting the transformed plants with the selection marker (ii); c) crossing a transformed plant with another plant belonging to a line containing in its genome a gene encoding an endogenous active transposase, and which is in the middle of a phenotypic marker for excision (iii), to obtain an F1 or any other individual of a subsequent generation; d) selecting the cells or the individuals carrying the gene of interest free of foreign ancillary sequence, from the F1 generation; e) regenerating plants from the cells or the individuals selected in (d).
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102649959-A
priorityDate 2001-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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