http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7498481-B2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ea8794e259cac44c8a1c099320a57560 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87 |
filingDate | 2003-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2009-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba9d8e7c9ae9f653ee49e27b6268b0f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b0f3565a545ea2c39e66c5ae9092e5f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a10be1a1c7ce74d0fd80c47799c0d8d |
publicationDate | 2009-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-7498481-B2 |
titleOfInvention | Method for obtaining a monocotyledon plant containing a gene of interest free of foreign ancillary sequence |
abstract | The present invention relates to a method for obtaining a transgenic monocotyledon plant containing a gene of interest free of foreign ancillary sequences comprising:n a) transforming at least one cell of a plant having no active transposase with a vector comprising two expression cassettes, one comprising a nucleotide sequence of interest (i), the other comprising a nucleotide sequence encoding a selection marker (ii) bordered by the mobilizable sequences of a transposon, said first expression cassette (comprising the nucleotide sequence of interest (i)) being outside the transposon element; b) selecting the transformed plants with the selection marker (ii); c) crossing a transformed plant with another plant belonging to a line containing in its genome a gene encoding an endogenous active transposase, and which is in the middle of a phenotypic marker for excision (iii), to obtain an F1 or any other individual of a subsequent generation; d) selecting the cells or the individuals carrying the gene of interest free of foreign ancillary sequence, from the F1 generation; e) regenerating plants from the cells or the individuals selected in (d). |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102649959-A |
priorityDate | 2001-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 123.