http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7456020-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5e5c121269689cbab9b3a4548b15caf3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate | 2003-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2008-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f5c23842dbff918931ce3298230b55e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ccdc600fbf9835a3ed8b9bdf097822c2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c09665fa20b33e16853611c5020d802e |
| publicationDate | 2008-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-7456020-B2 |
| titleOfInvention | Method for modifying chromosomes |
| abstract | The present invention provides a simple method for splitting and loss of a chromosome in yeast. The method for modifying a chromosome in yeast includes preparing a linear chromosome splitting vector (1) having a target sequence (a), a marker gene sequence and (C 4 A 2 ) n sequence in this order; preparing a linear chromosome splitting vector (2) having a target sequence (b), a centromere sequence of a yeast chromosome and (C 4 A 2 ) n sequence in this order; and introducing the chromosome splitting vectors (1) and (2) into yeast. Herein, n is each independently an integer of 6 to 10. Although this chromosome splitting vector has a repetitive sequence of 5′-CCCCAA-3′, it can be amplified specifically with PCR, so that a chromosome splitting vector can be prepared significantly simply and easily, compared with the conventional DNA splitting method. It seems that a yeast telomere sequence is bound to the (C 4 A 2 ) n sequence of the split chromosome generated by splitting with the chromosome splitting vector, and therefore the fragment can function as an intact chromosome. |
| priorityDate | 2002-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.