| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f79e0e1bc7b584c696e0810c1e235c3a |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K5-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-061 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00 |
| filingDate |
2004-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2008-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f22d6eb705a429681bbdd84da0ba22d3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d40f092b1dc4c8cbaa443c76c874bd32
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_666196c3242cc4d1d66ced006c6ec9ff |
| publicationDate |
2008-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-7414106-B2 |
| titleOfInvention |
Synthesis of peptide α-thioesters |
| abstract |
Disclosed herein is a new method for the solid phase peptide synthesis (SPPS) of C-terminal peptide α thioesters using Fmoc/t-Bu chemistry. This method is based on the use of an aryl hydrazine linker, which is totally stable to conditions required for Fmoc-SPPS. When the peptide synthesis has been completed, activation of the linker is achieved by mild oxidation. The oxidation step converts the acyl-hydrazine group into a highly reactive acyl-diazene intermediate which reacts with an α-amino acid alkylthioester (H-AA-SR) to yield the corresponding peptide α-thioester in good yield. A variety of peptide thioesters, cyclic peptides and a fully functional Src homology 3 (SH3) protein domain have been successfully prepared. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103755843-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10450343-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103755843-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10087221-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8242058-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109311941-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109311941-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008020942-A1 |
| priorityDate |
2003-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |