http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7413906-B2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c4322cb4de342be2dbedcbe0c278f6f |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-811 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2458-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-805 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6803 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-726 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6851 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
filingDate | 2006-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2008-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff802e183d7361d2fd92810859e1bc0b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06da63d56697ab3eef53a86ec7b2f753 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2df8fb6b2b357537d7dfc0b1cb9d48f7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27da31bc455db5982ed559da1428b50e |
publicationDate | 2008-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-7413906-B2 |
titleOfInvention | Quantitative, high-throughput screening method for protein stability |
abstract | In proteomic research, it is often necessary to screen a large number of polypeptides for the presence of stable structure. Described herein are methods (referred to as MALDI MS-HX and SUPREX) for measuring the stability of proteins in a rapid, high-throughput fashion. The method employs hydrogen exchange to estimate the stability of quantities of unpurified protein extracts, using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. A method of quantitatively determining the stability of a test protein under native conditions is disclosed. The method includes the steps (a) providing a test protein; (b) contacting the protein with an exchange buffer comprising a denaturant and deuterium, the exchange buffer having a denaturant concentration; (c) contacting the test protein with a mass spectrometry matrix medium; (d) determining a change in mass of the test protein by mass spectrometry; (e) varying the denaturant concentration of the exchange buffer; (f) repeating steps (a)-(e) a desired number of times; and (g) quantitatively determining protein stability based on the change in mass of the test protein as a function of denaturant concentration, whereby the stability of a test protein under native conditions is quantitatively determined. |
priorityDate | 2000-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 355.