http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7344864-B2

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filingDate 2003-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2008-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb476f252bdc433a6dd3e21380151951
publicationDate 2008-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-7344864-B2
titleOfInvention Drosophila clipped FRT (cFRT) chromosome insensitive to P transposage, generating method thereof, and application thereof
abstract A method for generating a Drosophila clipped FRT (cFRT) chromosome is provided, wherein the chromosome is insensitive to a P transposase but remains functional to a yeast site-specific flippase recombinase (FLP). The method includes steps of: (a) exposing a FRT chromosome to the P transposase for occurring a local and imprecise transposition, wherein the FRT chromosome contains a P[FRT] insertion with a selection marker gene, (b) screening the P[FRT] insertion insensitive to the P transposase to obtain screened products, (c) selecting candidate products from the screened products by further examinations, and (d) exposing the candidate products by the P transposase and selecting a desired product by the further examinations to obtain the Drosophila clipped FRT (cFRT) chromosome insensitive to the P transposase but remaining functional to the yeast site-specific flippase recombinase. The cFRT 2L2R chromosome can be used as the direct target in the direct P-transposon-induced mutagenesis.
priorityDate 2002-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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