Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5098eff6d67391cd857fae0aa3174bcb |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A21D8-042 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C11D3-386 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C11D3-38627 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A21D2-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C11D3-386 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A21D8-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L7-104 |
filingDate |
2006-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2007-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_71a935de5f541d4ce5a50118c55642d8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99f9aec73a9389c97cc82f5f66e46c01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a4a5e4ad0565a61255cf2bb923b3408 |
publicationDate |
2007-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-7271139-B2 |
titleOfInvention |
Lipolytic enzyme genes |
abstract |
The inventors have isolated novel lipolytic enzyme genes with a high homology to the T. lanuginosus lipase gene and thus well suited for use in gene shuffling. Accordingly, the invention provides a method of generating genetic diversity into lipolytic enzymes by family shuffling of two or more homologous genes which encode lipolytic enzymes. The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity. The invention also provides a polynucleotide comprising a nucleotide sequence encoding a lipolytic enzyme and a lipolytic enzyme (a polypeptide with lipolytic enzyme activity). |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8759044-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8765425-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3470504-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011078949-A1 |
priorityDate |
2001-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |