| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_22853c8669cb52e7d6fea1eea6467958 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1077
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0036
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-13 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 |
| filingDate |
2006-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2007-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7cdb40cd293d3bdac64c8a5c8a09b6eb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74e72408184a6f32eb108b5acd697669
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83b65dce727caa9f479a8b2c76e8c88f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_811aa283767987e15e8d921758f5faaf |
| publicationDate |
2007-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-7259242-B2 |
| titleOfInvention |
Methods for production of proteins |
| abstract |
The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli . The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8519099-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007190606-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8012715-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012149180-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10302591-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9409942-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8389681-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9733212-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007184527-A1 |
| priorityDate |
1997-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |