| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_436b489653124a7c40658a96293b471c |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-485 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
| filingDate |
2004-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2007-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10d5c227cbf12e8315e09cae8ab011b8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dfe987afb26b9d455ad870301af9f587
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b605e8d95f0e610813cb6f3461863956
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c82db3935f5dd581cc8b6b3dbf221e02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_186b42deda33ade4f38a9a33c5adf0cd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f02b01d498776287efbdb7041eaf9d4 |
| publicationDate |
2007-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-7255985-B2 |
| titleOfInvention |
Fluorine NMR spectroscopy for biochemical screening |
| abstract |
High-Throughput Screening (HTS) of large compound libraries is the method of drug-lead discovery. It is now well accepted that for a functional assay, quality is more important than quantity. A biochemical NMR method originally proposed by Percival and Withers (Biochemistry, 1992, 31, 498–505) is extended to the screening of Ser/Thr kinases. The method requires the presence of a CF 3 (or CF) moiety on the substrate and utilizes 19 F NMR spectroscopy for the detection of the starting and enzymatically modified substrates. Experiments can be performed in real time or in an endpoint assay format using protein and substrate concentrations comparable to the ones used by other HTS techniques. Application of this technique to the phosphorylation of a substrate by the protein Ser/Thr kinase AKT1 is presented. |
| priorityDate |
2003-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |