Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e6d4d7321e22e3b85d537f2b3f9b1f9b |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C49-788 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C49-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C45-455 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C225-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C255-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C49-813 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C49-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D213-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C51-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07F9-5345 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C255-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C225-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C51-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D213-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C49-813 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C49-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C49-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C49-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C45-45 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C49-788 |
filingDate |
2002-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2006-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4cc8aa0d7434237ad701aa3d82f4bf77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f808d85059b0da815d6d4bb72f72ac3c |
publicationDate |
2006-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-7053249-B2 |
titleOfInvention |
Metal chelates and methods of using them for time-resolved fluorescence |
abstract |
β-diketone fluorescent tags are disclosed, particularly those enabling the use of excitation energy in the near visible or visible spectrum. In some cases, these tags allow the use of cost-effective excitation devices such as LED's. The compounds form fluorescent chelates (complexes) with lanthanide (III) rare earth metal ions (such as Eu3+). The fluorescent complex may be included in a latex microparticle, such as a styrene latex particle. Ideally, the complex has an absorption maximum λ equal to or greater than 360 nm, and the compound is characterized by a pKa<9.0. Kits and methods for detecting target molecules (e.g. immunoassays) are also disclosed. Such methods and kits typically use a ligand for binding to the target molecule and a labeling agent attached to the ligand. The fluorescent complexes described above are at least part of the labeling agent. Apparatus for detecting fluorescence from a sample includes an irradiating energy source that produces irradiating energy λ equal to or greater than 360 nm; a detector positioned to detect fluorescence from the sample; and a sample holder for holding the sample in position to be irradiated by the energy source. A light-emitting diode is preferably used as the irradiating energy source. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101017168-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015241424-A1 |
priorityDate |
2002-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |