abstract |
The present invention provides for a column for use in capillary electrochromatography including a tube, wherein the tube includes an inner surface having a positively or negatively charged, chemically-bonded stationary coating thereon. The present invention further provides for a method of making a column for use in capillary electrochromatography, including a tube, wherein the tube includes an inner surface having a positively or negatively charged chemically-bonded stationary coating thereon, wherein the steps include filling the column with a sol-gel solution, maintaining the sol-gel solution within the column, and forcing the sol-gel solution out of the column with an inert gas. Further, the present invention provides for a method of analytical separation with a column including an inner layer having a positively charged chemically-bonded stationary coating thereon that reverses the direction of electroosmotic flow in the column compared with a column without the positive surface charge. Additionally, the present invention provides for a method of using a column including an inner layer of positively charged chemically-bonded stationary coating thereon by introducing a sample into the column operating under reversed electroosmotic flow due to positively charged chemically-bonded stationary coating. |