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filingDate 2002-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2006-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-6989271-B2
titleOfInvention Schwann cells originating in myeloid interstitial cells
abstract There is provided a method of inducing bone marrow stromal cells to differentiate into bone marrow stromal cell-derived Schwann cells in vitro, comprising the steps of: collecting bone marrow stromal cells from bone marrow and culturing the cells in a standard essential culture medium supplemented with a serum; adding a reducing agent to the culture medium and further culturing the cells; adding a differentiation inducing agent to the culture medium and further culturing the cells; and adding a cyclic AMP-augmenting agent or a cyclic AMP analogue and/or a glial cell differentiation and survival stimulating factor to the culture medium, and further culturing the cells to obtain the bone marrow stromal cell-derived Schwann cells. There are also provided bone marrow stromal cell-derived Schwann cells obtained thereby and a pharmaceutical composition for neural regeneration that comprises them.
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