| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2fc8c4f23758df7ed658973e2a913e38 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-146
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-12 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-805
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8261 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-12 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-805
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate |
1999-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2005-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_861edb61ca0492e4e07761355504d3c1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_00ae52c143fecf39c8f00811c83fa254
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3800675363989c6b267eec626d2dfa23
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b781a49c4bf1522633559074a0a9bbb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f6a92514fb16a76c1d0896026d53488e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_329925abb8f87637c607250f6a4be867 |
| publicationDate |
2005-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6936749-B1 |
| titleOfInvention |
Nonsymbiotic plant hemoglobins to maintain cell energy status |
| abstract |
Nonsymbiotic hemoglobins are broadly present across evolution; however, the function of these proteins is unknown. Cultured maize cells have been transformed to constitutively express a barley hemoglobin gene in either the sense (HB + ) or antisense (HB − ) orientation. Hemoglobin protein in the transformed cell lines was correspondingly higher or lower than in wild type cells under normal atmospheric conditions. Limiting oxygen availability, by placing the cells in a nitrogen atmosphere for 12 hours, had little effect on the energy status of cells constitutively expressing hemoglobin, but had a pronounced effect on both wild type and HB − cells, where ATP levels declined by 27% and 61% respectively. Energy charge was relatively unaffected by the treatment in HB + and wild type cells, but was reduced from 0.91 to 0.73 in HB − cells suggesting that the latter were incapable of maintaining their energy status under the low oxygen regime. Similar results were observed with P. aeruginosa cells transformed with an Hb expression vector. It is suggested that nonsymbiotic hemoglobins act to maintain the energy status of cells in low oxygen environments and that they accomplish this effect by promoting glycolytic flux through NADH oxidation, resulting in increased substrate level phosphorylation. Nonsymbiotic hemoglobins are likely ancestors of an early form of hemoglobin that sequestered oxygen in low oxygen environments, providing a source of oxygen to oxidize NADH to provide ATP for cell growth and development. This in turn suggests that cells containing increased levels of Hb protein will survive longer under low oxygen tension or high energy demand. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9700067-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11439166-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10798958-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9808029-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8084667-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9011949-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9826772-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11219232-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10172381-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10172380-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10039306-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11224241-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007256196-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10327464-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111286520-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010064384-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10863761-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10986848-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10993462-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10314325-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9943096-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11013250-B2 |
| priorityDate |
1998-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |