| abstract |
The present invention relates to a method of manufacturing a kit for isolating and purifying nucleic acids, or various biological materials, a kit manufactured by the method, and an apparatus using the kit. n The method of manufacturing a kit for isolating or purifying nucleic acids or biological materials from biological samples using solid materials comprises the steps of; fabricating a container having a plurality of chambers and/or column which is configured to contain the solid materials, buffers and/or enzymes suitable for isolation of the nucleic acids or biological materials; fabricating a cover including a protruded portion with a bore formed therethrough; filling each of the chambers or column with the solid materials, buffers and/or enzymes for isolation and purification of the biological materials or nucleic acids; sealing the container with a predetermined sealing material; and packaging the cover and the container, one by one, with a separate case. n According to the present invention, the kit, which is simple, inexpensive and effective in a small or medium number of sample manipulations, can be manufactured. Thus, manual pipetting works by a person who has not fully trained for clinical or biological experiments can be avoided. Further, the disposable kit having the column in which the nucleic acids isolated from the biological samples have been already contained can be directly transferred to the conventional PCR cycler in order to perform the PCR amplification without changing any other buffers and tubes. |