http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6864057-B2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a73cee5001ad08339ae751c10d9abf71 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2002-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2005-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_46cab75ed981e4454236e4329acf456d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_398338e10ea133087ebc49435cb34256 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cd7472ede4441a312af4a7a8faf39ce3 |
publicationDate | 2005-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-6864057-B2 |
titleOfInvention | Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing |
abstract | Methods are provide for preparing cDNA corresponding to a mRNA. In the subject methods, a mRNA is first contacted with a mixture of primers under first strand cDNA synthesis conditions. The primer mixture contains primers that have at least 10 contiguous deoxythymidines, a double stranded restriction enzyme recognition sequence near one end and a non-polyA-complementary region near the other end, where the non-polyA-complementary region is -VV, -VTV, -VTTV, -VTTTV, and -VVVVV. The resultant cDNA is modified such that the polyT tail is substantially removed. The modified cDNA is then ligated into a vector. The subject methods find use in a variety of applications, and find particular use in the sequencing of DNA and in the synthesis of cDNA libraries. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006160116-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011015085-A1 |
priorityDate | 2000-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 498.