| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5717a86578bcc50cd5de72d43c10f2c2 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-118339
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-117497
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-405
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-10 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N13-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-648
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-03 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-34 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-05
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-03
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-27
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-43
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N13-00 |
| filingDate |
1999-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2005-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79bfdf469ead8d80e1084523ecf05255
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d597c55e3ce3e44f49afa5ca70c3ae80 |
| publicationDate |
2005-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6843963-B1 |
| titleOfInvention |
Flow-through shear analyzer for biologically active molecules in liquid layers on surfaces |
| abstract |
The adsorption rate of proteins from solutions on surfaces in the region of interface layers is often so large that a depletion of the protein in the interface layer results. Due to this, the total reaction becomes transport-dependent, sensitively disrupting the determination of the rate constants. In known TIRF-analysis chambers or bio-sensor systems with a liquid interface layer of ˜10 μm thickness and mass transport coefficients of 10 −6 -10 −5 m/s it has up limitation. n With the help of a TIRF-flow-through shear analyzer in which a certain volume unit of an immiscible fluid, for example an air bubble, is fed into the buffer flow, an ultra-thin liquid layer arises on the surface with a thickness of 100-200 nm, wherein interface surfaces below 10 nm thickness are technically possible. n The new TIRF-flow-through shear analyzer therefore allows the generation of ultra-thin liquid layers while increasing the mass transport coefficients for proteins by 50-100-fold so that the sorption rate constants can be determined without transport limitation. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9958319-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102010041426-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007062583-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102012102983-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011284769-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007039866-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109580482-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7955864-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11408817-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2018021238-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8362449-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007141593-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9285297-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007202609-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011171748-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007079196-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007079196-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3495802-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007068573-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8361807-B2 |
| priorityDate |
1998-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |